Intended use: The kit can be used for basic amplification of DNA templates, the designed primers and DNA templates can be added to amplify the target fragment, and the amplified products can be detected by agarose gel electrophoresis, etc.
DNA Isothermal Rapid Amplification Kit Storage and term of Validity
Storage term: stored at ≤-20℃，keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3' end of the primer to start the chain extension.
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Suitable for DNA isothermal rapid amplification kit(Basic type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 37 to 44℃ and time is 20-30 minutes.