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Isothermal nucleic acid amplification test Applicable equipment:
It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.
Storage and term of Validity
Storage term: stored at ≤-20℃，keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3' end of the primer to start the chain extension. The kit relies on the role of exonuclease and adds the designed specific molecular probes according to the template at 39℃, and achieves the real-time monitoring of amplification process of the target fragment.
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
MIRA fluorescent probe principle
Pseudorabies virus (PRV) test project: gradient dilution and detection after nucleic acid extraction from swab samples.
Staphylococcus aureus detection project: gradient dilution and detection after bacterial nucleic acid extraction.
