Intended use: The kit can be used for Colloidal gold strip test of RNA templates, the designed primers, probes and DNA templates can be added for amplification and the amplified products can be used for colloidal gold strip color reaction.
isothermal fluorescence pcr Applicable equipment:
It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.
isothermal amplification rpa Storage and term of Validity
Storage term: stored at ≤-20℃，keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
The kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: under constant temperature 42℃, the specially modified reverse transcriptase with specific primer DNA and template RNA synthesize cDNA strand, and the recombinase, single-stranded DNA binding protein and DNA polymerase in the reaction system carry out rapid nucleic acid amplification by the template of newly synthesized cDNA strand. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
SARS-CoV-2 colloidal gold test strips Inspection (N gene)
Reaction condition: 42 ℃, 10mins, color development 3-5 mins after reaction
Template: pseudovirus nucleic acid extraction accounting decuple gradient dilution