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Porcine epidemic diarrhea virus detection kit -exo
Specification: 48 Tests/Packing
Intended use: The kit can be used for fluorescence amplification of DNA templates, the designed primers, probes and DNA templates can be added to carry out multienzyme rapid fluorescence detection.
Product Detail

Porcine epidemic diarrhea virus detection kit -exo Applicable equipment

It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.

Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing

Term of Validity: 14 months

Principle Summary

The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3' end of the primer to start the chain extension. The kit relies on the role of exonuclease and adds the designed specific molecular probes according to the template at 39℃, and achieves the real-time monitoring of amplification process of the target fragment.


1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Porcine epidemic diarrhea virus detection kit -exo Product Features

1/ High sensitivity and specificity, short reaction time

2/ The reagent form is freeze-dried, stable and easy to operate

Porcine epidemic diarrhea virus detection kit -exo Applications:

African swine fever virus detection

Samples: gradient dilution and detection after nucleic acid extraction from swab samples

Temperature: 39℃

Reaction Time: 20 mins

Sensitivity: Nucleic acid diluted to 10-6 (qPCR Ct 38.783(30copies/mL))

Amplification Plot

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