Intended use: The kit can be used for basic amplification of RNA templates, the designed primers and RNA templates can be added to amplify the target fragment, and the amplified products can be detected by agarose gel electrophoresis, etc.
isothermal rna amplification Applicable equipment:
It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.
Storage and term of Validity
Storage term: stored at ≤-20℃，keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
The kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: under constant temperature 42℃, the specially modified reverse transcriptase with specific primer DNA and template RNA synthesize cDNA strand, and the recombinase, single-stranded DNA binding protein and DNA polymerase in the reaction system carry out rapid nucleic acid amplification by the template of newly synthesized cDNA strand. The kit is suitable for laboratory RNA amplification and RNA amplification for other detection purposes.
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
Suitable for RNA isothermal rapid amplification kit(Basic);
Primer: Require pair of nucleotide primers with the length of 25-35 bp;
RNA basic kit reaction temperature is 42 to 44℃ and time is 20-30 minutes.
RNA Basic Kit single isothermal amplification result