Intended use: The kit can be used for fluorescence amplification of RNA templates, the designed primers, probes and RNA templates can be added to carry out multienzyme rapid fluorescence detection.
isothermal nucleic acid amplification method Applicable equipment:
It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.
Storage and term of Validity
Storage term: stored at ≤-20℃，keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
The kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: under room and constant temperature, the specially modified reverse transcriptase with specific primer DNA and template RNA synthesize cDNA strand, and the recombinase, single-stranded DNA binding protein and DNA polymerase in the reaction system carry out rapid nucleic acid amplification by the newly synthesized cDNA strand template. The kit relies on the role of exonuclease and adds the designed specific molecular probes according to the template at 42℃，achieves the real-time monitoring of amplification process of the target fragment. And it is suitable for laboratory RNA amplification and RNA amplification for other detection purposes.
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
Cat detection project: gradient dilution and detection after nucleic acid extraction of blood sample.
Porcine Reproductive and Respiratory Syndrome Virus(PRRSV) detection
project: gradient dilution and detection after swab sample