About MIRA
The Principle of MIRA
Multienzyme Isothermal Rapid Amplification(MIRA) is a multi-enzyme isothermal nucleic acid rapid amplification technology. MIRA relies on the synergy of multiple functional proteins at room temperature to achieve rapid nucleic acid amplification. It is a truly portable on-site rapid nucleic acid detection technology.
● At constant room temperature, recombinase and primers form protein/single-stranded nucleotide complex: the Rec/ssDNA;
● The complex search the DNA double strands for homologous sequences. After finding the target region, the complex invades the double-stranded DNA template within the helper protein and single-stranded binding protein (SSB), and form the D-loop at the invasion site;
● The Rec/ssDNA complex disintegrates and the polymerase binds to the 3 'end of the primer to initiate chain extension.
A diagram showing the structure of RNA (left) and DNA (right). Uracil is the base paired with adenine in RNA, whereas thymine is paired with adenine in DNA. Image credit: Wikipedia
Features of MIRA
FastResults can be obtained in 5-20 min, realizing a real "quick test".
SimpleFreeze-dried and Microsphere reagent, easy to operate with portable equipment, at constant room temperature.
PreciseHigh sensitivity and specificit.
DiverseThe results are presented in various ways: electrophoresis, real-time fluorescence, test strips, etc.
Comparison of Molecular Detection Techniques
MIRA | PCR | LAMP | |
Principles of Technology | Multienzyme Isothermal Rapid Amplification | Polymerase Chain Reaction | Loop-mediated isothermal amplification |
functional enzyme | single-stranded binding protein, polymerases, recombinases, etc. | DNA polymerase | Bst DNA polymerase |
Reaction temperature | 25-45℃ constant temperature | 95 ℃-55 ℃-72 ℃ Variable temperature | 65 ℃ constant temperature |
Reaction time | 5-20 mins | 1.5-2 h | 40-60 mins |
Primers | 2 | 2 | 4-6 |
Reagent state | Liquid /Freeze-dried /microspheres | Liquid | Liquid |
Equipment | Thermostatic equipment(metal bath, water bath, etc.) | PCR instrument | Thermostatic equipment |
Operability | The operation is extremely simple, and it can realize the real portability | Professional operation requirements are high, and equipment operation is complicated | Simple operation |
Aerosol pollution | Amplification at room temperature to reduce the risk of aerosol contamination | High temperature, risk of aerosol contamination | High temperature, risk of aerosol contamination |
The Principle of MIRA
Multienzyme Isothermal Rapid Amplification(MIRA) is a multi-enzyme isothermal nucleic acid rapid amplification technology. MIRA relies on the synergy of multiple functional proteins at room temperature to achieve rapid nucleic acid amplification. It is a truly portable on-site rapid nucleic acid detection technology.
● At constant room temperature, recombinase and primers form protein/single-stranded nucleotide complex: the Rec/ssDNA;
● The complex search the DNA double strands for homologous sequences. After finding the target region, the complex invades the double-stranded DNA template within the helper protein and single-stranded binding protein (SSB), and form the D-loop at the invasion site;
● The Rec/ssDNA complex disintegrates and the polymerase binds to the 3 'end of the primer to initiate chain extension.
A diagram showing the structure of RNA (left) and DNA (right). Uracil is the base paired with adenine in RNA, whereas thymine is paired with adenine in DNA. Image credit: Wikipedia
Features of MIRA
FastResults can be obtained in 5-20 min, realizing a real "quick test".
SimpleFreeze-dried and Microsphere reagent, easy to operate with portable equipment, at constant room temperature.
PreciseHigh sensitivity and specificit.
DiverseThe results are presented in various ways: electrophoresis, real-time fluorescence, test strips, etc.
Comparison of Molecular Detection Techniques
MIRA | PCR | LAMP | |
Principles of Technology | Multienzyme Isothermal Rapid Amplification | Polymerase Chain Reaction | Loop-mediated isothermal amplification |
functional enzyme | single-stranded binding protein, polymerases, recombinases, etc. | DNA polymerase | Bst DNA polymerase |
Reaction temperature | 25-45℃ constant temperature | 95 ℃-55 ℃-72 ℃ Variable temperature | 65 ℃ constant temperature |
Reaction time | 5-20 mins | 1.5-2 h | 40-60 mins |
Primers | 2 | 2 | 4-6 |
Reagent state | Liquid /Freeze-dried /microspheres | Liquid | Liquid |
Equipment | Thermostatic equipment(metal bath, water bath, etc.) | PCR instrument | Thermostatic equipment |
Operability | The operation is extremely simple, and it can realize the real portability | Professional operation requirements are high, and equipment operation is complicated | Simple operation |
Aerosol pollution | Amplification at room temperature to reduce the risk of aerosol contamination | High temperature, risk of aerosol contamination | High temperature, risk of aerosol contamination |
