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Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
Kit composition (WLRE8208KIT 48T/Pack)
1 mL×2 Tubes
Positive control template
|Product features and advantages
Specificity is superior to PCR
|Short reaction time
Liquid, freeze-dried powder, freeze-dried ball
|Easy to operate
Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the results
Design of primers and probe is simple
|Easy to store and transport
It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.
The freeze-dried form has a long storage time and is convenient for transportation
Low requirements on equipment
Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;
Applicable to our WL-16-III and other isothermal fluorescence detector
African Swine Fever Virus Isothermal Detection
Operation procedure and process:
Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix.
Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent.
Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid
Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control
Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap)
Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone.
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