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14 Months Storage Isothermal PCR Amplification Kit High Sensitivity Specificity

14 Months Storage Isothermal PCR Amplification Kit High Sensitivity Specificity

14 Months Storage pcr amplification kit

pcr amplification kit Isothermal

High Sensitivity isothermal nucleic acid

Place of Origin:

China

Brand Name:

Amp-future bio

Certification:

ISO9001

Model Number:

WLRN8211KIT

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Product Details
Application:
RNA Nucleic Acid Amplification
Components:
Primers, Taq DNA Polymerase, DNTPs, Mg2+
Detection Method:
NFO
Instrument:
Test Strips
Kit Size:
48 Reactions
Product Format:
Lyophilized
Product Name:
RNA Isothermal Amplification NFO Kits
Product Type:
Kit
Reaction Time:
5-20 Minutes
Reaction Volume:
50μl
Shelf Life:
14 Months
Shipping Condition:
Ice
Storage Temperature:
-20°C
Target Template:
RNA
Payment & Shipping Terms
Minimum Order Quantity
48T
Price
USD3.8$/T
Packaging Details
16T/Bag,48T/Box
Delivery Time
6days
Payment Terms
T/T paypal
Supply Ability
100000T/Month
Product Description

Isothermal PCR Kit - High Sensitivity & Specificity, 14 Months Storage

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

 

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3' end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

 

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

 

Technical Parameters:
Parameters Details
Product Name RNA Isothermal Amplification Kit NFO
Manufacturer Amp-future
Storage Temperature -20°C
Kit Components Enzymes, Buffers ,Reagents
Packaging 48 Tests/box
Detection Limit 500-1000copies/µL
Shipping ICE
Test Time 5-20mins

 

Isothermal nucleic acid Applications

Suitable for RNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

 

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

 

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