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20mins DNA Isothermal Nucleic Acid Amplification Kit For High Speed PCR

20mins DNA Isothermal Nucleic Acid Amplification Kit For High Speed PCR

DNA isothermal nucleic acid amplification

20mins isothermal nucleic acid amplification

pcr isothermal Kit

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Product Details
Reaction Time:
Kit Type:
Reaction Volume:
50 μL
Shelf Life:
2 Years
Payment Method:
Credit Card/PayPal/TT
Primers, Polymerase, Buffer, DNTPs
Product Name:
DNA Isothermal Amplification Kit Basic
Storage Temperature:
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Product Description

Accurate And Cost-Effective DNA Amplification Kit For High-Speed PCR

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months


Isothermal nucleic acid Principle Summary

The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3' end of the primer to start the chain extension.


Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.


Technical Parameters:
Parameters Details
Product Name DNA Isothermal Amplification Kit Basic
Manufacturer Amp-future
Storage Temperature -20°C
Kit Components Enzymes, Buffers ,Reagents
Packaging 48 Tests/box
Detection Limit 500-1000copies/µL
Shipping ICE
Test Time 5-20mins


Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(Basic type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42 and time is 5-20 minutes.



1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.


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