DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

【Principle Overview】

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3' end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

【Primer design】

It is recommended to use primers with a length of 30-35 bp.Too short primers will affect the amplification speed and detection sensitivity;the 5' end of the reverse primer is labeled with a modification group (commonly used biotin).

Primers are designed to avoid the formation of secondary structures that affect amplification; the length of the amplicon is recommended to be 150-500bp.

【Colloidal gold probe design】

In the middle of the forward and reverse primer,design a sequence of 46-52nt in length that is complementary to the target fragment; modify an antigen marker (typical FAM) at the 5' end;mark a dSpacer (tetrahydrofuran, THF) in the middle of the 5' end and the 3' end ), as the recognition site of nfo;the 3' end is labeled with a modification group, such as amine group, phosphate group or C3-Spacer, etc.

【Features】

This kit has the advantages of high sensitivity, strong specificity,and short reaction time (only 15 minutes),and the reaction components are in dry powder state,which is easy to operate and easy to store.

This reagent has low equipment requirement,and the reaction operation can be carried out in metal baths, water baths, etc.,and there is no need to purchase expensive exclusive equipment such as PCR amplifiers.

【Kit storage】

1. Storage conditions: storage temperature ≤ -20℃constant temperature environment, keep away from light and avoid heavy pressure;

2. Product validity period: 14 months;

3. See the outer packaging for the production date.

Experimental operation video, please contact sales to obtain it for free