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One Step Isothermal Basic RNA Amplification Kit For High Throughput RNA Analysis

One Step Isothermal Basic RNA Amplification Kit For High Throughput RNA Analysis

Basic rna amplification kit

Isothermal rna amplification kit

One Step Isothermal PCR Kit

Place of Origin:

China

Brand Name:

Amp-future bio

Certification:

ISO9001

Model Number:

WLRB8207KIT

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Product Details
Format:
Lyophilized
Compatibility:
Universal
Storage Temperature:
-20°C
Product Name:
RNA Isothermal Amplification Basic Kit
Sensitivity:
High
Amplification Time:
5-20mins
Application:
RNA Nucleic Acid Amplification
Reaction Volume:
50 μL
Highlight:

Basic rna amplification kit

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Isothermal rna amplification kit

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One Step Isothermal PCR Kit

Payment & Shipping Terms
Minimum Order Quantity
48T
Price
USD3.8$/T
Packaging Details
16T/Bag,48T/Box
Delivery Time
6days
Payment Terms
T/T paypal
Supply Ability
100000T/Month
Product Description

     One-Step Isothermal Amplification Basic Kit for High Throughput RNA Analysis

 

  Product Detail

Kit Storage and Term of Validity

 

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

 

Isothermal Nucleic Acid Principle Summary

 

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature:at room temperature and constant temperature(generally 39℃~42℃),reverse transcriptaseuses specific primers and template RNA tosynthesize cDNA strands,and binds the auxiliary protein and single strand with the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology positionand strand exchange begins; accompanied by the recombinase from the complex upon dissociation,the polymerase also bindsto the 3' end of the primer,initiating chainextension.It is suitable for laboratory-level RNA amplification and RNA amplification for other detection purposes.

 

Technical Parameters:
Parameters Details
Product Name RNA Isothermal Amplification Kit Basic
Manufacturer Amp-future
Storage Temperature -20°C
Kit Components Enzymes, Buffers ,Reagents
Packaging 48 Tests/box
Detection Limit 500-1000copies/µL
Shipping ICE
Test Time 5-20mins

 

Isothermal Nucleic Acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

 

Isothermal Nucleic Acid Applications

Suitable for RNA isothermal rapid amplification kit(BASIC type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA basic kit reaction temperature is 39 to 42 and time is 5-20 minutes.

 

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

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